The efficiency of Poly(ADP-Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion.

Show simple item record Aslan Koşar, Pınar Tuncer, Hamdi Cihangir Uğuz, Abdülhadi Espino Palma, Javier Darıcı, Hakan Onaran, İbrahim Çiğ, Bilal Koşar, Alim Rodriguez Moratinos, Ana Beatriz 2021-06-22T07:28:43Z 2021-06-22T07:28:43Z 2015
dc.identifier.issn 0959-9673
dc.identifier.other 26303136
dc.identifier.uri 10.1111/iep.12137 en_US
dc.description.abstract The aim of this study was to evaluate the histopathological and apoptotic changes occurring in the rat ipsilateral and contralateral testes, after experimental spermatic cord torsion, and to explore and the role of poly(ADP-ribose) polymerase (PARP) cleavage in testicular torsion-detorsion injury. A total of 37 Wistar albino rats were subjected to 720° unilateral spermatic cord torsion for 1, 2 and 4 h, followed by 4-h reperfusion, or else to a sham operation (control group). Histology of the testicle was evaluated using haematoxylin-eosin (H&E) staining and Johnsen's scoring system. Germ cell apoptosis was evaluated via active caspase-3 immunostaining, and PARP expression levels were evaluated via Western blotting. The mean Johnsen's tubular biopsy scores (JTBS) of the ipsilateral testicles were lower for all torsion groups than for the controls (P < 0.05), but the JTBS of the contralateral testicles were only lower in the 4-h torsion group (P < 0.05). The mean apoptosis score (AS) of the ipsilateral and contralateral testicles was significantly higher in the torsion groups than in the sham group. AS increased correlatively with torsion time, in both testicles. The effect of testicular torsion on PARP cleavage was time dependent, with the highest effect observed after 4 h of testicular torsion (P < 0.05). Testicular torsion caused time-dependent histological changes, apoptosis and increases in PARP cleavage. Our results suggest that testicular torsion-detorsion injury caused cell damage and germ cell apoptosis that apparently involved cleavage of PARP. Increased PARP cleavage could, in turn, lead to enhanced apoptosis. en_US
dc.language.iso English en_US
dc.publisher WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject apoptosis en_US
dc.subject poly(ADP-ribose) polymerase (PARP) en_US
dc.subject testis torsion en_US
dc.title The efficiency of Poly(ADP-Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion. en_US
dc.type Article en_US
dc.identifier.issue 5 en_US
dc.identifier.startpage 294 en_US
dc.identifier.endpage 300 en_US
dc.identifier.volume 96 en_US
dc.contributor.authorID 0000-0001-9393-554X : Hakan Darıcı en_US
dc.contributor.authorID 0000-0001-7832-066X : Bilal Çiğ en_US
dc.contributor.authorID 0000-0001-6063-0504 : Ana Beatriz Rodríguez Moratinos en_US
dc.contributor.authorID 0000-0003-2602-5145 : Pınar Aslan Koşar en_US
dc.contributor.authorID 0000-0002-8549-9343 : Espino Javier en_US
dc.identifier.wos 000367324300003 en_US
dc.identifier.doi 10.1111/iep.12137 en_US
dc.contributor.sankoauthor Hakan Darıcı en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Gazimuhtar Paşa Bulvarı
Şehitkamil / GAZİANTEP