Effects of bleomycin and antioxidants on the fatty acid profile of testicular cancer cell membranes.

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dc.contributor.author Cort, Aysegul
dc.contributor.author Ozben, Tomris
dc.contributor.author Melchiorre, Michele
dc.contributor.author Chatgilialoglu, Chryssostomos
dc.contributor.author Ferreri, Carla
dc.contributor.author Sansone, Anna
dc.date.accessioned 2021-06-24T12:14:07Z
dc.date.available 2021-06-24T12:14:07Z
dc.date.issued 2016
dc.identifier.issn 0005-2736
dc.identifier.other 26656160
dc.identifier.uri http://openaccess.sanko.edu.tr/xmlui/handle/20.500.12527/266
dc.description.abstract Bleomycin is used in chemotherapy regimens for the treatment of patients having testicular germ-cell tumor (TGCT). There is no study in the literature investigating the effects of bleomycin on membrane lipid profile in testicular cancer cells. We investigated membrane fatty acid (FA) profiles isolated, derivatized and analyzed by gas chromatography of NTera-2 testicular cancer cells incubated with bleomycin (Bleo) for 24 h in the absence and presence of N-Acetyl-L-Cysteine (NAC) and curcumin (Cur) as commonly used antioxidant adjuvants. At the same time the MAPK pathway and EGFR levels were followed up. Bleomycin treatment increased significantly saturated fatty acids (SFA) of phospholipids at the expense of monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA). Bleomycin also led to a significant increase in the trans lipid isomers of oleic and arachidonic acids due to its free radical producing effect. Incubation with bleomycin increased the p38 MAPK and JNK levels and downregulated EGFR pathway. Coincubation of bleomycin with NAC reversed effects caused by bleomycin. Our results highlight the important role of membrane fatty acid remodeling occurring during the use of bleomycin and its concurrent use with antioxidants which can adjuvate the cytotoxic effects of the chemotherapeutic agents. en_US
dc.language.iso English en_US
dc.publisher ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Bleomycin en_US
dc.subject Curcumin en_US
dc.subject Membrane lipid profile en_US
dc.subject N-acetyl-L-cysteine en_US
dc.subject Testicular cancer cell en_US
dc.title Effects of bleomycin and antioxidants on the fatty acid profile of testicular cancer cell membranes. en_US
dc.type Article en_US
dc.relation.journal BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES en_US
dc.identifier.issue 2 en_US
dc.identifier.startpage 434 en_US
dc.identifier.endpage 441 en_US
dc.identifier.volume 1858 en_US
dc.identifier.wos 000369559600030 en_US
dc.contributor.sankoauthor Ayşegül Çört en_US


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Gazimuhtar Paşa Bulvarı
No:36
27090
Şehitkamil / GAZİANTEP