dc.contributor.author | Sarı, Ayşe Nur | |
dc.contributor.author | Süzük, Serap | |
dc.contributor.author | Karatuna, Onur | |
dc.contributor.author | Öğünç, Dilara | |
dc.contributor.author | Karakoç, Ayşe Esra | |
dc.contributor.author | Çizmeci, Zeynep | |
dc.contributor.author | Alışkan, Hikmet Eda | |
dc.contributor.author | Cömert, Füsun | |
dc.contributor.author | Bakıcı, Mustafa Zahir | |
dc.contributor.author | Akpolat, Nezahat | |
dc.contributor.author | Çilli, Fatma Feriha | |
dc.contributor.author | Zer, Yasemin | |
dc.contributor.author | Karataş, Aysel | |
dc.contributor.author | Akgün Karapınar, Bahar | |
dc.contributor.author | Bayramoğlu, Gülçin | |
dc.contributor.author | Özdamar, Melda | |
dc.contributor.author | Kalem, Fatma | |
dc.contributor.author | Delialioğlu, Nuran | |
dc.contributor.author | Aktaş, Elif | |
dc.contributor.author | Yılmaz, Nisel | |
dc.contributor.author | Gürcan, Şaban | |
dc.contributor.author | Gülay, Zeynep | |
dc.date.accessioned | 2021-07-13T11:45:04Z | |
dc.date.available | 2021-07-13T11:45:04Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0374-9096 | |
dc.identifier.other | 28929967 | |
dc.identifier.uri | http://openaccess.sanko.edu.tr/xmlui/handle/20.500.12527/340 | |
dc.description.abstract | Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmid-mediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae isolates from different parts of our country. A total of 329 Enterobacteriaceae isolates from 22 laboratories were collected which were isolated between March, 2015 and February, 2016. mcr-1/mcr-2 were investigated by polymerase chain reaction during February-March, 2016. Two hundred and seventeen of Klebsiella pneumoniae (66%), 75 of Salmonella spp. (22.8%), 31 of Esherichia coli (9.4%), 3 of Enterobacter cloacae (0.9%), 2 of Klebsiella oxytoca (0.6%) and 1 of Enterobacter aerogenes (0.3%) isolates were included to the study. Agarose gel electrophoresis results of PCR studies have shown expected band sizes for positive control isolates as 309 bp for mcr-1 and 567 bp for mcr-2. However, the presence of mcr-1/mcr-2 genes was not detected among the tested study isolates of Enterobacteriaceae. Although mcr-1/mcr-2 were not detected in our study isolates, it is highly important to understand the mechanism of resistance dissemination and determine the resistant isolates by considering that colistin is a last-line antibiotic against infections of multidrug or carbapenem resistant gram-negative bacteria. Thus, it is suggested that these mechanisms should be followed-up in both clinical and non-clinical (e.g. isolates from food animals, raw meats and environment) isolates of special populations. | en_US |
dc.language.iso | Turkish | en_US |
dc.publisher | ANKARA MICROBIOLOGY SOCHACETLEPE UNIV FACULTY MEDICINE DEPT MICROBIOLOGY, 06100 ANKARA, TURKEY | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Colistin | en_US |
dc.subject | mcr-1 | en_US |
dc.subject | mcr-2 | en_US |
dc.subject | Enterobacteriaceae | en_US |
dc.title | [Results of a multicenter study investigating plasmid mediated colistin resistance genes (mcr-1 and mcr-2) in clinical Enterobacteriaceae ısolates from Turkey]. | en_US |
dc.title.alternative | Ülkemizde Klinik Enterobacteriaceae İzolatlarında Plazmit Aracılı Kolistin Direnç Genlerini (mcr-1 ve mcr-2) Araştıran Çok Merkezli Çalışmaya Ait Sonuçlar | en_US |
dc.type | Article | en_US |
dc.relation.journal | MIKROBIYOLOJI BULTENI | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 299 | en_US |
dc.identifier.endpage | 303 | en_US |
dc.identifier.volume | 51 | en_US |
dc.contributor.authorID | 0000-0002-3927-9921 : Ayşe Nur Sari Kaygisiz | en_US |
dc.identifier.wos | 000408311400011 | en_US |
dc.identifier.doi | 10.5578/mb.57515 | en_US |
dc.contributor.sankoauthor | Ayşe Nur Sarı | en_US |
Gazimuhtar Paşa Bulvarı
No:36
27090
Şehitkamil / GAZİANTEP