dc.contributor.author | Özdemir, Evrim Dursun | |
dc.contributor.author | Hanikoglu, Aysegul | |
dc.contributor.author | Cort, Aysegul | |
dc.contributor.author | Ozben, Beste | |
dc.contributor.author | Suleymanlar, Gultekin | |
dc.contributor.author | Ozben, Tomris | |
dc.date.accessioned | 2021-07-14T08:00:37Z | |
dc.date.available | 2021-07-14T08:00:37Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1899-5276 | |
dc.identifier.other | 28691421 | |
dc.identifier.uri | https://doi.org/ 10.17219/acem/62834 | en_US |
dc.identifier.uri | http://openaccess.sanko.edu.tr/xmlui/handle/20.500.12527/344 | |
dc.description.abstract | Atherosclerosis and atherosclerosis-related complications are the main cause of death in the world. Vascular injury in response to inflammation and enhanced oxidant stress promotes endothelial dysfunction and leads to atherosclerotic lesions. Low-dose treatment with darbepoetin-α may be a potential therapeutic tool for endothelial injury and atherosclerosis. In order to study the effect of darbepoetin-α on endothelial injury and atherosclerosis, we used ApoE-/- mice as the atherosclerotic mice model. We monitored atherosclerosis and plaque formation histochemically in ApoE knockout mice at early and late stages of atherosclerosis. Darbepoetin-α was injected intraperitoneally at a dose of 0.1 μg/kg to ApoE-/- mice. The results of 2 ApoE-/- mice groups injected with darbepoetin-α (early and late stages of atherosclerosis) were compared to the results of the corresponding saline injected ApoE-/- mice groups and the control (C57BL/6) mice. Lipid profile (total cholesterol, triglyceride), inflammation (CRP, IL-6, histamine), endothelial injury (ICAM-1, selectin) and oxidative stress markers (lipid peroxidation, protein oxidation) were significantly increased in 4 atherosclerotic groups compared to the control group. Short-term darbepoetin-α had no marked effects on indicators of inflammation and endothelial injury in the ApoE knockout mice groups compared to the ApoE knockout mice not treated with darbepoetin-α, however, darbepoetin-α significantly decreased 8-isoprostane and protein carbonyl content. Long term darbepoetin-α treatment reduced oxidative stress in ApoE-/- mice. This study contributes to understanding and elucidating the biochemical changes occurring during early and late stages of atherosclerosis development regarding lipid profile, inflammation, endothelial injury and oxidative stress markers. | en_US |
dc.language.iso | English | en_US |
dc.publisher | WROCLAW MEDICAL UNIVUL K MARCINKOWSKIEGO 2-6, WROCLAW, 50-368, POLAND | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | ApoE knockout mice | en_US |
dc.subject | atherosclerosis | en_US |
dc.subject | darbepoetin-α | en_US |
dc.subject | endothelial dysfunction | en_US |
dc.subject | oxidative stress | en_US |
dc.title | Effects of long- and short-term darbepoetin-α treatment on oxidative stress, inflammation and endothelial injury in ApoE knockout mice. | en_US |
dc.type | Article | en_US |
dc.relation.journal | ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.startpage | 635 | en_US |
dc.identifier.endpage | 643 | en_US |
dc.identifier.volume | 26 | en_US |
dc.contributor.authorID | 0000-0002-1413-4523 : Tomris Ozben | en_US |
dc.contributor.authorID | 0000-0001-7935-6402 : Gultekin Suleymanlar | en_US |
dc.contributor.authorID | 0000-0002-3484-6392 : Beste Ozben | en_US |
dc.identifier.wos | 000408663700012 | en_US |
dc.identifier.doi | 10.17219/acem/62834 | en_US |
dc.contributor.sankoauthor | Ayşegül Çört | en_US |
Gazimuhtar Paşa Bulvarı
No:36
27090
Şehitkamil / GAZİANTEP