dc.contributor.author | Gök Yurtseven, Duygu | |
dc.contributor.author | Serter Koçağlu, Sema | |
dc.contributor.author | Minbay, Zehra | |
dc.contributor.author | Eyigör, Özhan | |
dc.date.accessioned | 2021-10-20T17:33:02Z | |
dc.date.available | 2021-10-20T17:33:02Z | |
dc.date.issued | 2020-09-11 | |
dc.identifier.other | 32932902 | |
dc.identifier.other | 2076-3425 | |
dc.identifier.uri | http://openaccess.sanko.edu.tr/xmlui/handle/20.500.12527/465 | |
dc.description.abstract | Nesfatin-1, identified as an anorexigenic peptide, regulates the energy metabolism by suppressing food intake. The majority of nesfatin-1-synthesizing neurons are concentrated in various hypothalamic nuclei, especially in the supraoptic (SON), arcuate (ARC) and paraventricular nuclei (PVN). We tested the hypothesis that the glutamatergic system regulates nesfatin-1 neurons through glutamate receptors. Therefore, the first aim of the proposed studies was to examine effects of different glutamate agonists in the activation of nesfatin-1 neurons using c-Fos double immunohistochemical labeling. Experimental groups were formed containing male and female rats which received intraperitoneal injections of glutamate agonists kainic acid, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) while the control rats received vehicle. The significant increase in the number of c-Fos-expressing nesfatin-1 neurons after agonist injections were observed both in female and male subjects and some of these effects were found to be sexually dimorphic. In addition, treatment with specific glutamate antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) or dizocilpine (MK-801) before each of the three agonist injections caused a statistically significant reduction in the number of activated nesfatin-1 neurons in the hypothalamic nuclei including supraoptic, paraventricular and arcuate nuclei. The second aim of the study was to determine the expression of glutamate receptor subunit proteins in the nesfatin-1 neurons by using a double immunofluorescence technique. The results showed that the glutamate receptor subunits, which may form homomeric or heteromeric functional receptor channels, were expressed in the nesfatin-1 neurons. In conclusion, the results of this study suggest that nesfatin-1 neurons respond to glutamatergic signals in the form of neuronal activation and that the glutamate receptors that are synthesized by nesfatin-1 neurons may participate in the glutamatergic regulation of these neurons. | en_US |
dc.language.iso | English | en_US |
dc.publisher | MDPIST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | c-Fos | en_US |
dc.subject | glutamate | en_US |
dc.subject | hypothalamus | en_US |
dc.subject | nesfatin-1 | en_US |
dc.subject | rat | en_US |
dc.title | Immunohistochemical Evidence for Glutamatergic Regulation of Nesfatin-1 Neurons in the Rat Hypothalamus. | en_US |
dc.type | Article | en_US |
dc.relation.journal | BRAIN SCIENCES | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.volume | 10 | en_US |
dc.contributor.authorID | 0000-0001-5757-8450 : Zehra Minbay | en_US |
dc.contributor.authorID | 0000-0003-3463-7483 : Özhan Eyigör | en_US |
dc.identifier.wos | 000580138200001 | en_US |
dc.identifier.doi | 10.3390/brainsci10090630 | en_US |
dc.contributor.sankoauthor | Duygu Gök Yurtseven | en_US |
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Gazimuhtar Paşa Bulvarı
No:36
27090
Şehitkamil / GAZİANTEP