Brusatol Mitigates Ovarian Tissue Oxidatif Injury Induced by Ovarian Ischemia Reperfusion

Abstract

Objective: In the scope of this study, it was tried to examine the probable benefits of brusatol againstovarian tissue injury originating from bilateral ovarian torsion/detorsion (T/D).Material and Methods: In this study, experimental animals were randomized to 4 groups. Groupswere programmed as sham group I (control), group II (T/D), group III (DMSO+T/D) and groupIV (Brusatol+T/D). Group I; the abdomen region was barbered, disinfected, opened by incision andclosed respectively, but no T/D model was performed. Group II, as described in group I, after openingand closing abdominal incision, 3 hours of torsion was applied and it was followed by a 3-hour ofdetorsion. Group III, 1% DMSO was applied intraperitoneally 0,3 ml as every other day for 10days and the last dose was given 30 minutes prior to detorsion. Group IV; brusatol was administeredintraperitoneally 0,5 mg/ml once every 2 days for 10 days and the last dose was applied 30 minutesprior to detorsion. Then, all processes were carried out as described in group II. Following detorsionperiod, in pursuit of sacrificing the rats, ovarian tissues were removed.Results: Oxidant parameters (MDA, MPO, TOS, OSI) and pro-inflammatory cytokine (TNF-α,IL-1β) levels increased significantly while antioxidant levels (SOD, TAS) reduced in group II and IIIcompared to group I (p<0.05). On the contrary, brusatol application (group IV) reversed all of thesevalues compared to group II and III (p<0.05).Conclusion: As a conclusion, brusatol was evaluated to have protective effects against T/D-inducedovarian injury in rats.